Fig. 2.

Knockdown of lncRNA 4930581F22Rik impedes skeletal muscle differentiation. (A) Quantitative real-time PCR analysis evaluated the knockdown efficiency of lncRNA4930581F22Rik with three different siRNAs. (B) The morphology changes of myoblasts that transfected with siRNAs. (C) Effects of 4930581F22Rik knockdown on MyHC expression and myotube formation. Scale bar: 100 μm. (D) Effect on differentiation index and fusion index after knockdown of 4930581F22Rik. ns: no significant difference. Values were presented as means ± SEM (n = 3). The statistical significance of differences between the two groups was calculated using unpaired t-test.