Fig. 4.

lncRNA 4930581F22Rik regulates skeletal muscle differentiation through the ERK/MAPK signaling pathway. (A) Western blot analysis reveals the protein expression levels of p-ERK and ERK in myoblasts transfected with siRNAs. (B) Quantification of protein expression for p-ERK and ERK in myoblasts transfected with siRNAs. (C) Western blot analysis reveals the protein expression levels of p-JNK, JNK, p-38 and P38 in myoblasts transfected with siRNAs. (D) Quantification of protein expression for p-JNK, JNK, p-38 and P38 in myoblasts transfected with siRNAs. GAPDH was used as a loading control. The molecular weight of the targeting protein is indicated as the marker (MK) showed. ns: no significant difference. Values were presented as means ± SEM (n = 3). The statistical significance of differences between the two groups was calculated using unpaired t-test.