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. 2024 May 6;10(9):e30640. doi: 10.1016/j.heliyon.2024.e30640

Fig. 5.

Fig. 5

A negative regulation between lncRNA 4930581F22Rik and ERK/MAPK pathway. (A) Quantitative real-time PCR analysis was performed to assess the expression levels of MAPK1 and MAPK3 in myoblasts transfected with siRNAs. (B) Western blot analysis shows the expression levels of c-Raf protein in myoblasts transfected with siRNAs. (C) Western blot analysis shows the expression levels of c-Raf protein in myoblasts transfected with siRNAs. (D) Luciferase reporter assay showing the effects of lncRNA 4930581F22Rik knockdown on the activity of ERK1/2 signaling pathway. (E) Western blot analysis of ERK, p-ERK, MyHC, and MEF2C in myoblasts transfected with siRNAs that were treated with 2 μM PD0325901 or DMSO. (F) Western blot analysis of ERK, p-ERK, MyHC and MEF2C in myoblasts transfected with siRNAs that were treated with 10 μM PD0325901 or DMSO. (G) Relative expression in F were calculated. The molecular weight of the targeting protein is indicated as the marker (MK) showed. ns: no significant difference. Values were presented as means ± SEM (n = 3). The statistical significance of differences between the two groups was calculated using unpaired t-test.