Fig 1.

Comparison of HSAF production in a series of potential c-di-GMP receptor protein deletion mutants. The HSAF production in all strains was detected by HPLC. The peak area corresponding to HSAF in the wild-type strain OH11 was assumed to be 1, and all other strains were compared to the wild type. Statistical comparisons were performed with GraphPad software (GraphPad, La Jolla, CA) using one-way ANOVA (Dunnett’s multiple comparisons test). The error bars indicate standard errors. ****P < 0.0001, relative to the wild-type OH11. Biological experiments for each treatment were performed three times and assayed in triplicate.