Fig 2.

LspE is a T2SS ATPase and a c-di-GMP receptor. (A) The protease activity of the wild-type and LspE mutants. Statistical comparisons were performed with GraphPad software (GraphPad, La Jolla, CA) using one-way ANOVA (Dunnett’s multiple comparisons test). The error bars indicate standard errors. *P ≤ 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, relative to the wild type. Biological experiments for each treatment were performed three times and assayed in triplicate. (B) In vitro ATP hydrolysis activity assay of LspE-Hcp1 protein. (C) SPR experiments were performed to evaluate the binding ability of c-di-GMP with LspE-Hcp1 with or without ATP-gamma-S.