Fig 5.

The ATPase activity of LspE is closely correlated with HSAF production. (A) Mutation R298, R313, R424, and R457 of LspE, respectively, significantly reduces the ATP hydrolysis ability compared to the wild type. Statistical comparisons were performed with GraphPad software (GraphPad, La Jolla, CA) using one-way ANOVA (Dunnett’s multiple comparisons test). The error bars indicate standard errors. ****P < 0.0001, relative to the LspE-Hcp1. Biological experiments for each treatment were performed three times and assayed in triplicate. (B) Point mutations of the four R residues in L. enzymogenes OH11 genome severely impair the ability of the strain to produce HSAF. Statistical comparisons were performed with GraphPad software (GraphPad, La Jolla, CA) using one-way ANOVA (Dunnett’s multiple comparisons test). The error bars indicate standard errors. ****P < 0.0001, relative to the wild-type OH11. Biological experiments for each treatment were performed three times and assayed in triplicate.