TABLE 2.

Quantitative PCR summary table with primer and probe sequences for the novel Lp-p6 (ΔRn= 0.35) and previously published LG-ITS assay (ΔRn= 0.60) (44) and results for in silico and in vitro specificity for L. petauri

	LG-ITSa	Lp-p6
Forward primer	5′-CAA GAT AGA GAA GAT TGC GTT GAG-3′	5′-CGT CTT CCA TAT TCT GAA ATT TGT ATC AGG-3′
Reverse primer	5′-CCG TAT CTT ATG GAG CCT AGC-3′	5′-GCC AAT TCT ACC AAT GCT AAC AC-3′
Probe	5′-FAM- TGC TTT GCA CGC AGG AGG TCA -BHQ-3′	5′-NED- AGC GTA AGG TAG AGC TTT CTT GTG CC -NFQ-3′
In silico binding
L. formosensis	5/5	0/5
L. garvieae	7/7	0/7
L. petauri	87/87	80/87
Other Lactococcus	0/13	0/13
DNA amplification
L. formosensis	4/4	0/4
L. garvieae	8/8	0/8
L. petauri	26/26	26/26
Other Lactococcus	0/21	0/21

^a Shahin et al. (44).