Figure 1.
Lectin microarray analyses of rAAV6 produced by different methods
(A) Representative glycan profiles of six rAAV6 preparations produced by different methods. The plot shows the mean-normalized signal intensities with standard diviation (SD) from triplicate spots of the 42 lectins for 2.5 × 109 vg. The signals of O-glycan recognition lectins, ACG, ABA, Jacalin, and ACG, are indicated for all samples; N-glycan recognition lectins are additionally indicated for samples 4 and 5. (B and C) PCA score (B) and loading plot (C) based on the glycan profiles obtained by lectin microarray analyses. Different samples are represented with different colors. The colors of the dots in the PCA score plot correspond to the bars in the glycan profiles of each sample. The number of each dot corresponds to the sample information used for the PCA analysis shown in Table S1. Representative N-glycan-recognized lectins are shown in blue and O-glycan-recognized lectins in red in the PCA loading plot.