Glycopeptide analysis of rAAV6 by LC-MS/MS with CID fragmentation
(A) Schematic of identified glycans and glycosylation sites. O-GlcNAc is depicted as a blue square, O-GalNAc as a yellow square, galactose as a yellow circle, and sialic acid as a magenta diamond. (B) The CID mass spectrum of T162–K168 modified with HexNAcHexNeuAc2. (C) The CID mass spectrum of V239–R245 modified with HexNAc.