Figure 6.

A structural model of rAAV6 glycosylation

(A) A structural model of the VP2 and VP3 region of rAAV6 modified with O-GlcNAc and O-GalNAc. The crystal structure of AAV6 VP3 (PDB: 3OAH) was used for A218–L736, whereas A139–A217 was derived from the VP2 structure, as modeled by AlphaFold2.⁴¹ The glycans were attached by CHARMM-GUI Glycan Modeler.⁴²O-GlcNAc is colored blue, O-GalNAc in magenta, the VP2 region in orange, the modeled VP3 in green, and VP3 from the crystal structure in gray. (B) A structural model of rAAV6 capsid based on the crystal structure of AAV6 VP3 (PDB: 3OAH). A whole capsid is shown on the left and a sliced-through capsid on the right. The residues colored blue are T242, which was identified as an O-GlcNAc attachment site.