Fig. 6.

Enrichment pathways of HMGB2 and co-expression protein analysis.(A) Enriched DDR pathways by GSEA based on CGGA-mRNAseq_325 dataset.(B)PPI between HMGB2 and the proteins of enriched DDR pathways constructed by the STRING and cytoscape visualization software. (C-D)Co-expression analysis of HMGB2 with APEX1 and HMGB1 with CGGA-mRNAseq_325 dataset.(E-F)The expression levels of APEX1 and HMGB1 in glioma and non-glioma tissues in GBM and LGG with TCGA datebase. (G-H)In the TCGA database, the survival differences between the high expression and low expression groups of HMGB1 and APEX1.(I)Expression of HMGB2 proteins in glioma cell lines (U87,U251,U118,U138,LN229,T98G,Hs683)by western blotting.(J-K)WB verification of HMGB2 knock-down and overexpression.(L-M)WB verification was performed after HMGB2 overexpression cells were added with solvent and OLA.(N,P)Verification of HMGB1 expression in HMGB2 overexpression and knockdown cells.(O,Q)HMGB1 expression histogram with prism8.