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. 2017 Apr 8;74(16):3057–3064. doi: 10.1007/s00018-017-2521-1

Fig. 5.

Fig. 5

5mC labelling on the condensed chromatin changes moving towards the nuclear envelope. The chromatin appears less stained after EDTA technique. At the surface of the heterochromatin (zone 3), the gold grains (12 nm) are abundant gradually decreasing in the zone 2; nearby the nuclear envelope (zone 1) they are almost absent. Rat liver; 250 nm