Fig. 6.

BDNF/pCREB signaling enhanced by CDK5 shRNA-miR. Primary hippocampal neurons were transduced with Scr (black bars) or CDK5 shRNA-miR at DIV7 (gray bars). DIV18 neurons were treated with 125-μM glutamate for 20 min, and evaluated 24-h post-glutamate. a BDNF protein levels for each treatment were measured through BDNF Emax immunoassay system. The data are presented as the mean ± SEM from n = 5, performed in duplicate. The values were normalized to the control. b Representative blots and c quantification of TrkB (FL and T), pERK1/2 (ratio to ERK), pCAMKII and pCREB (ratio to CREB), and p35 and CDK5 protein levels were measured by western blot, and were normalized each βIII-tubulin by fluorescence intensity analysis on the bar graph as arbitrary units (RU), n = 4; *p < 0.05, **p < 0.01, ***p < 0.001; Kruskal–Wallis’ test with Dunn’s for a and c