Fig. 6.

FRET of the amyloidogenic and aggregated states of M-TTR. a Effect of different NaCl concentrations on the kinetics of 15 µM DACM-M-TTR aggregation in 20 mM acetate buffer, pH 4.4, 37 °C, monitored with turbidimetry at 450 nm. b DLS size distributions of M-TTR and DACM-M-TTR at both pH 7.4 and 4.4. c, d Fluorescence spectra at different time points for M-TTR (c) and DACM-M-TTR (d) in 20 mM acetate buffer, 30 mM NaCl, pH 4.4, 37 °C. e Change of E during aggregation. Conditions as in c, d. f FRET E values for native M-TTR at pH 7.4, 25 °C (first bar), amyloidogenic monomeric M-TTR at pH 4.4, 37 °C, 0 s (second bar) and aggregated M-TTR at pH 4.4, 37 °C, 3000 s (third bar)