Fig. 9.

RET of W79F-M-TTR during refolding. a, b Refolding time course of W79F-M-TTR (red filled circles) and DACM-W79F-M-TTR (blue open circles) monitored by tryptophan fluorescence (excitation 290 nm, emission 320–385 nm) in 0.5 M urea at pH 7.4, 25 °C. c Refolding time course of DACM-W79F-M-TTR (blue open circles) monitored by DACM fluorescence (excitation 290 nm, emission >385 nm) in 0.5 M urea at pH 7.4, 25 °C. d Comparison between the FRET E values calculated, from the spectroscopic data, for the folded (F), unfolded (U) and partially folded (PF) states of M-TTR and W79F-M-TTR in 0.5 M urea at pH 7.4, 25 °C