Fig. 3.

Effect of TcdB on EGC viability and cell death. Control EGCs and EGCs treated with TcdB (0.1–10 ng/ml) were recovered at 1.5–48 h, and the percentage of trypan blue+ cells (a) or the total cell number (b) was determined using trypan blue. Data are the mean ± standard deviation of six experiments performed in triplicate. *P < 0.01 TcdB-treated EGCs versus control EGCs, Student’s t test. ^§ P < 0.01 TcdB-treated EGCs at 24 or 48 h versus control EGCs at time 0, Student’s t test