Fig. 9.

TcdB activates caspase-3 but not caspase-9 in EGCs. Lysates from control EGCs and EGCs treated with TcdB (0.1–10 ng/ml) prepared at 1.5–48 h were subjected to SDS-PAGE. The filters were probed with: a anti-caspase-9 then stripped and probed with anti-β-actin; b anti-β-actin then stripped and probed with anti-caspase-3. The graphs represent the densitometric analysis of each protein relative to β-actin. *P < 0.01 TcdB-treated EGCs versus control EGCs