Fig. 1.

Retinoic acid inhibits human myoblast differentiation. Human myoblasts were induced to differentiate in differentiation medium for 4 days in the presence of increasing concentrations of RA or not (ctrl) for the first 48 h (refreshed after 24 h). a Representative images (six fields for each condition) showing myogenin (red) and Troponin T (green) expression. DNA was stained with DAPI (blue). b Quantification of the percentage of myogenin-positive nuclei relative to all cell nuclei and c quantification of the average area of myotubes (in μm²) in the cultures, as shown in a. p ≤ 0.05 (*) and p ≤ 0.01 (**). Scale bars 10 μM