Fig. 5.
RA-signalling pathway is activated in quiescent mouse satellite cells. a Graph showing GFP-positive satellite cells isolated by FACS from adult Tg: Pax7-nGFP mice (n = 3). b RT-qPCR analysis of the expression of myogenic determination, differentiation and proliferation genes, some RA-target genes, RA receptors, and genes encoding proteins involved in RA biogenesis in quiescent and activated mouse satellite cells. c Percentage of EdU, MyoD, and myogenin-positive nuclei relative to all cell nuclei in activated mouse satellite cells treated (RA) or not (ctrl) with 10−7M RA or 10−6M RA for indicated days. p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***)