Fig. 2.

Transmitter-elicited reduction of cytosolic calcium responses is transient and CREB-dependent. Astrocytes were treated with 10 µM NA to induce CREB activation. Representative traces of calcium responses induced by 100 µM ATP at 12 h (a) and 24 h (b) after CREB stimulation. c Quantification of peak calcium responses induced by ATP after 6, 12, and 24 h after CREB stimulation. The data are the mean ± SEM of 3–4 independent experiments. *P < 0.05, ***P < 0.001. d, e Representative 100 µM ATP-induced calcium traces in single cells and f quantification of calcium responses after 6 h of stimulation of CREB with 10 µM NA in Null and A-CREB-infected astrocytes. The data are the mean ± SEM of 3–4 independent experiments. **P < 0.01