Fig. 3.

VP16-CREB decreases calcium responses induced by transmitters. a VP16 immunocytochemistry and b western blot of VP16 in astrocytes infected with VP16-CREB or Null viral vectors. c Schematic representation of the protocol used to measured calcium responses in astrocytes infected with VP16-CREB or Null viral vectors. Representative single cell traces of calcium responses induced by 100 µM ATP (d) or 10 µM NA (f) in VP16-CREB-infected astrocytes (virus MOI 1). e, g Quantification of peak calcium responses at different viral vector loads. Data are normalised for the response of Null-infected astrocytes at the same viral vector load. The data are the mean ± SEM of 4–7 (d) or 3–4 independent experiments (f). *P < 0.05, **P < 0.01