Fig. 5.

VP16-CREB increases mitochondrial calcium uptake. a Representative traces in single cells and b quantification of intracellular calcium responses induced by 100 µM ATP in Null and VP16-CREB-infected astrocytes in astrocytes treated with FCCP to inhibit mitochondrial calcium uptake. The data are the mean ± SEM of four independent experiments. c Representative images of the astrocytes transfected with the mitochondrial calcium dye CEPIA3mt before and after 100 µM ATP application. d Representative calcium traces and e quantification of 100 µM ATP-induced mitochondrial calcium increase in Null and VP16-CREB-infected astrocytes. The data are the mean ± SEM of four independent experiments. *P < 0.05