Fig. 6.

Sigma-1 receptor mediates the effects of VP16-CREB. a Quantification of sigma-1 receptor mRNA expression by quantitative PCR and b representative western blot of sigma-1 receptor in astrocytes infected with Null or VP16-CREB. c Representative calcium traces in single cells and d quantification of 100 µM ATP-induced ER calcium decreases measured using G-CEPIAer in astrocytes infected with null or VP16-CREB viral vectors and in the absence and presence of BD1047, a sigma-1 receptor antagonist. e Representative calcium traces in single cells and f quantification of 100 µM ATP-induced mitochondrial calcium increases using CEPIA3mt in astrocytes infected with Null or VP16-CREB viral vectors and in the absence and presence of BD1047. The data are the mean ± SEM of 3–4 independent experiments. *P < 0.05, ****P < 0.0001