Fig. 8.

CREB decreases calcium responses and upregulates sigma-1 receptor in adult astrocytes. a Representative traces and b quantification of calcium responses induced by 100 µM ATP after 6 h of CREB stimulation (1 h) with 10 µM NA or vehicle (control). c Representative traces and d quantification calcium responses induced by 100 µM ATP in adult cultured astrocytes infected with Null or VP16-CREB viral vectors. e Quantification of sigma-1 receptor mRNA expression by quantitative PCR in Null and VP16-CREB-infected adult cultured astrocytes. In (b, d, and e), data are the mean ± SEM of 4 independent experiments. *P < 0.05. f Quantification by quantitative PCR of sigma-1 receptor mRNA expression in cortices in WT and VP16-CREB mice before (WT and VP16-CREB) and after cryolesion (WT C and VP16-CREB C). Cryolesions were performed to increase VP16-CREB expression in astrocytes conditional to gliosis. Data are the mean ± SEM of 4–5 animals in each condition. *P < 0.05, **P < 0.01, ****P < 0.0001