Table 2.
Complementation of E. coli conditional strains by yodM
| Strain | CFU numeration | |||||
|---|---|---|---|---|---|---|
| −IPTG | +IPTG 1 mM | +IPTG 0.1 mM | ||||
| 30 °C | 42 °C | 30 °C | 42 °C | 30 °C | 42 °C | |
| BWTsbacA | 142 | 0 (0%) | 129 | 0 (0%) | 190 | 152 (80%) |
| BWPGPTs | 401 | 404 (100%) | 566 | 0 (0%) | 502 | 426 (85%) |
| BW25113 | 48 | 46 (96%) | 55 | 0 (0%) | 36 | 47 (130%) |
The two E. coli thermosensitive strains were transformed with the pTrcHis30:yodM plasmid and aliquots were plated onto two ampicillin-containing 2YT agar plates incubated at either 30 °C or 42 °C. The CFU were counted after 24 h incubation. Values in parentheses are ratios between the numbers of clones obtained at 42 °C and 30 °C.