Fig. 6.

TgNup407 localization is perturbed in the TgNup302-deficient parasites. a TgNup407 protein was tagged using a Myc-tag in the TgNup302-HA iKD strain. Endogenous TgNup302 iKD was labeled with rabbit monoclonal anti-HA (in red) and endogenous TgNup407 was labeled with mouse monoclonal anti-Myc (in green) antibody with or without ATc after 48 h of growth. b Measurement of cytoplasmic and nuclear Myc signals was determined for endogenous TgNup503 (horizontal striped bars), TgNup407 (empty bars) potential partners without ATc treatment (blue), and after 48-h ATc treatment (red). The ratio of the cytoplasmic signal versus nuclear signal was determined. The results shown are from three independent experiments. ***P < 0.0001