Fig. 4.

Capillary electropherograms showing the fragment sizes of cfDNA isolated from HepG2 cells after 4–72 h of incubation following medium renewal. Each shows a typical ladder pattern associable with nucleosomal subunits of predictable sizes that are most prominent at around 150–200, 300–400, and 500–600 bp. After 12 h, a major peak is visible at around 2000 bp. This peak reaches a maximum of a little under 80 FU at 24 h, but then decreases, while the smaller peaks rise