Fig. 5.

a ZO-1 and occludin expression in human Caco-2 cells after lentiviral depletion of CerS2. Western blot analysis of ZO-1 (1:1000) and occludin (1:500) in membrane fractions isolated from confluent grown CaCo-2 cells. Values are presented as mean ± SD, *p < 0.05 of three independent replicates. b Ex vivo FITC dextran permeability assay of CerS2^−/− and CerS2^+/+ mice colons. Permeability of intestinal sacs was calculated by measurement of FITC dextran diffusion from the sac lumen into the outer medium. FITC-Dex. permeability of each sac is shown for untreated CerS2^+/+ and CerS2^−/− mice. Significance of means was examined with unpaired t test. Data are mean ± SEM