Table 1.
RNA levels of CerS1-6 and glycosylceramide synthase (GCS) in colons of CerS2+/+ and CerS2−/− mice with and without treatment of AOM and two cycles of DSS [data are the mean ± SD and mice in each group (n)]
| mRNA | CerS2+/+ control | CerS2+/+ DSS | CerS2−/− control | CerS2−/− DSS | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean | SD | n | Mean | SD | n | Mean | SD | n | Mean | SD | n | |
| CerS1 | 1.01 | 0.35 | 5 | 249.1 | 362.0 | 12 | 4.55 | 5.12 | 6 | 16.24 | 16.21 | 4 |
| CerS2 | 13.16 | 24.31 | 5 | 60.95 | 72.33 | 12 | 0.84 | 0.57 | 6 | 4.11 | 2.68 | 4 |
| CerS3 | 2.21 | 1.95 | 6 | 91.82 | 147.8 | 12 | 4.39 | 3.14 | 6 | 18.19 | 28.27 | 4 |
| CerS4 | 12.29 | 14.22 | 6 | 12.31 | 17.19 | 13 | 11.98 | 12.45 | 6 | 9.89 | 10.80 | 4 |
| CerS5 | 3.44 | 3.90 | 6 | 9.24 | 7.77 | 13 | 5.48 | 4.35 | 6 | 8.12 | 9.59 | 4 |
| CerS6 | 7.64 | 9.70 | 6 | 10.52 | 15.92 | 15 | 5.31 | 7.51 | 6 | 3.84 | 6.67 | 4 |
| GCS | 1.15 | 0.70 | 3 | 10.55 | 12.33 | 10 | 0.51 | 0.23 | 3 | 1.17 | 1.55 | 2 |
Significance of means was examined by one-way ANOVA with post hoc Turkey’s correction and revealed no significant changes; therefore, trends are indicated in different fonts (“bold” indicates that the level is upregulated in comparison to CerS2+/+ control mice, “italics” indicates that the level is downregulated in comparison to CerS2+/+ control mice)