Table 2.
Sphingolipid levels determined by LC–MS/MS in colon and blood
| ng/mg tissue | CerS2+/+ control | CerS2+/+ DSS | CerS2−/− Control | CerS2−/− DSS | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean | SD | n | Mean | SD | n | Mean | SD | n | Mean | SD | n | |
| Sphingolipids colon | ||||||||||||
| Sphingosine | 0.22 | 0.07 | 7 | 0.25 | 0.22 | 24 | 0.33 | 0.10 | 5 | 0.23 | 0.10 | 11 |
| Sphinganine | 0.11 | 0.06 | 7 | 0.13 | 0.11 | 24 | 0.42 | 0.11 | 5 | 0.35 | 0.29 | 11 |
| S1P | 0.03 | 0.01 | 3 | 0.04 | 0.02 | 19 | 0.02 | 0.01 | 2 | 0.04 | 0.02 | 5 |
| GluCer 16:0 | 1.62 | 0.95 | 7 | 2.45 | 4.53 | 24 | 1.56 | 0.57 | 5 | 3.67 | 3.24 | 11 |
| C16:0-Cer | 10.43 | 5.53 | 7 | 7.84 | 6.01 | 22 | 13.43 | 5.56 | 5 | 6.44 | 2.46 | 11 |
| C14:0-Cer | 0.12 | 0.08 | 7 | 0.06 | 0.06 | 24 | 0.09 | 0.03 | 5 | 0.04 | 0.03 | 11 |
| C18:0-Cer | 1.92 | 1.05 | 7 | 1.75 | 0.93 | 24 | 2.58 | 1.11 | 5 | 1.21 | 0.39 | 11 |
| C20:0-Cer | 2.39 | 1.06 | 7 | 2.24 | 1.33 | 24 | 2.59 | 0.74 | 5 | 1.49 | 0.46 | 11 |
| C24:0-Cer | 1.12 | 0.69 | 6 | 1.41 | 1.19 | 24 | 0.45 | 0.10 | 3 | 0.53 | 0.40 | 6 |
| C24:1-Cer | 3.38 | 2.66 | 6 | 3.43 | 2.17 | 24 | 0.45 | 0.10 | 3 | 1.53 | 0.40 | 6 |
| C16:0-sphinganine | 0.91 | 0.39 | 7 | 0.97 | 0.96 | 24 | 1.98 | 0.85 | 5 | 1.14 | 0.39 | 11 |
| C18:0-sphinganine | 0.09 | 0.06 | 7 | 0.09 | 0.07 | 24 | 0.12 | 0.06 | 5 | 0.16 | 0.10 | 11 |
| C24:1-sphinganine | 0.16 | 0.06 | 6 | 0.22 | 0.19 | 24 | ND | ND | 5 | ND | ND | 6 |
| ng/ml plasma | CerS2+/+ control | CerS2+/+ DSS | CerS2−/− Control | CerS2−/− DSS | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean | SD | n | Mean | SD | n | Mean | SD | n | Mean | SD | n | |
| Sphingolipids plasma | ||||||||||||
| Sphingosine | 12.10 | 3.67 | 8 | 12.54 | 2.91 | 7 | 25.63 | 10.45 | 9 | 24.20 | 17.43 | 8 |
| Sphinganine | 10.19 | 5.81 | 8 | 11.81 | 2.62 | 7 | 80.03 | 47.06 | 9 | 42.94 | 31.52 | 8 |
| S1P | 479.75 | 152.60 | 8 | 312.46 | 63.99 | 7 | 1055.86 | 470.28 | 9 | 389.65 | 58.27 | 8 |
| SA1P | 157.5 | 72.92 | 8 | 74.31 | 9.20 | 7 | 468.90 | 66.18 | 4 | 370.56 | 184.35 | 8 |
| GluCer 16:0 | 262.76 | 410.64 | 8 | 670.03 | 557.45 | 7 | 13545 | 8543.9 | 9 | 2334.36 | 1931.64 | 8 |
| C16:0-Cer | 40.83 | 18.16 | 3 | 60.70 | 27.84 | 6 | 264.60 | 171.81 | 4 | 167.47 | 93.69 | 3 |
| C20:0-Cer | 49.96 | 26.12 | 8 | 99.39 | 48.64 | 7 | 26.37 | 18.46 | 9 | 42.08 | 45.79 | 8 |
| C24:0-Cer | 735.79 | 338.85 | 8 | 600.81 | 156.69 | 7 | 65.01 | 72.46 | 8 | 9.9 | 17.73 | 8 |
| C24:1-Cer | 470.75 | 295.36 | 8 | 625.90 | 151.99 | 7 | 39.66 | 72.56 | 9 | ND | ND | 8 |
Data are mean ± SD. Significance of means was examined by one-way ANOVA with post hoc Turkey’s correction. p < 0.05. Significant changes of sphingolipid levels in untreated CerS2−/− mice in comparison to untreated CerS2+/+ mice are shown in bold font