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Germline deletion of dgcr8 disrupts canonical microRNA function. a Q-PCR analysis of pri-miRNA and mature miRNA expression in embryos from the wild-type and MZdgcr8 mutant line at 6 hpf. Data are expressed as the mean ± SEM (n = 4). b miR-430 efficiently suppresses a dsRED reporter containing three imperfect targets (IPT) in the wild-type embryos but not in the MZdgcr8 mutant at 24 hpf
