Fig. 5.

Crb controls nephrocyte function by regulating endocytosis. a Endocytosed RFP accumulates in Rab7-positive vesicles, but does not show overlap with Rab5 (higher magnification in a′). b Rab7-labeled late endosomes and Rab5-positive early endosomes are decreased in nephrocytes with downregulated DmCrb (b, same exposure settings as in a, b′ shows optimized settings) in contrast to control nephrocytes (a). c RFP-accumulation assay of garland nephrocytes with downregulated expression of Expanded (Ex), Moesin (Moe), or Yurt (Yrt). d Nephrocytes expressing Moesin–RNAi show decreased Rab5 and to some extent lower Rab7 staining (same exposure settings as in a, d′ shows optimized settings). e Table summarizing the phenotypes observed in nephrocytes with Crb-knock-down and expression of indicated rescue constructs. Scale bars 1.5 µm in a′ and 5 µm in all other