Extended Data Fig. 5 |. Associations between prevalent bacterial taxa and tumor response in murine models.

a, Experimental setting for murine studies shown in Figs. 2 and 3. Treatment of established transplantable tumors (MCA205 sarcoma or RET melanoma) by intraperitoneal (i.p.) administrations of CICB and feces collection at three time points for 16S rRNA gene sequencing. Feces collection time points: T0=before treatment initiation (Day 0), T2=48 hours after 2 treatments (Day 5), T5=48 hours after 5 treatments (Day 14). In studies utilizing antibiotic (ATB) treatment, ATB was commenced 14 days prior to tumor inoculation and continued throughout. b, Pearson correlation between the relative abundance of Parabacteroides distasonis (at T0, T2, and T5) and standardized tumor size at T5 in MCA205 and RET tumor-bearing mice (two-sided p = 0.010, r = −0.614). c, Heatmap of Spearman correlations between the most prevalent (>20%) bacterial species identified in mouse feces at different time points (T0, T2, T5) from RET tumor-bearing mice and colon inflammatory infiltrates. Data are derived from combined discovery and validation cohort animals. Red represents a positive correlation, while blue represents a negative correlation with colonic infiltrate score. Following FDR adjustment, no significant correlations were observed.