Fig. 2.

Knockdown of TUFM induces EMT. a TUFM-knockdown-induced morphologic changes in lung cancer cell lines. Cells were photographed at ×200 magnification. Scr, scramble control (shRNA against a scrambled sequence). b TUFM-knockdown-induced F-actin rearrangement was detected by immunofluorescent staining. c Protein levels of EMT marker proteins ZO-1, E-cadherin, fibronectin, N-cadherin, vimentin, Snail were examined by immunoblotting. d Immunofluorescent staining of E-cadherin and fibronectin in A549 cells. e Relative mRNA levels of Slug, Twist, and Zeb2 in control and TUFM-knockdown A549 cells were quantified using real-time PCR. f Levels of E-cadherin and γ-catenin in MCF7 cells were examined by immunoblotting