Fig. 4.

TUFM knockdown reduces cell proliferation and xenograft tumor growth. a Cell proliferation was examined by BrdU incorporation assay. The inhibitory effect of TGF-β (5 ng/ml, 24 h) on CCL64 cells was used as a positive control. Data shown represent statistical analysis of three independent experiments. b The percentage of cells in different cell cycle phases was examined using FACS, and statistical analysis is shown. c, d At 7 weeks after subcutaneous injection of control or TUFM-knockdown A549 cells, nude mice were photographed (c), and tumors from each mouse were surgically isolated (d). e Control and TUFM-knockdown tumors were weighed after surgical isolation. Data shown are the statistical analysis of three independent experiments. f The diameters of control and TUFM-knockdown tumors were measured at the indicated time points. Statistical data of three independent experiments are presented