Fig. 5.

The cancer-associated CRM1 mutation E571K does not abrogate SRV100 export. a Schematic representation of the CRM1 protein illustrating the position of the recurrent cancer mutation E571K (blue star) proximal to the NES-binding cleft. b View of CRM1 NES-binding cleft highlighting wild type (green) and mutant (orange) versions of residues 571 (left panels) and 572 (right panels). An NES peptide is depicted in pink using ribbon representation. c Images show representative examples of 293T cells co-expressing SRV100 with either wild type YFP-CRM1 or with the indicated mutants. The graph indicates the percentage of co-transfected cells showing predominantly nuclear (N > C), nuclear and cytoplasmic (N = C) or predominantly cytoplasmic (C > N) localization of SRV100. At least 200 cells were counted per sample