Fig. 1.

Dissociation of cPLA₂α–AnxA1 induced by Shiga toxin. a HeLa cells were treated with 1 µg/ml ShigaB-sulf2 (StxB-S2) or 1.5 µg/ml Shiga-like toxin 1 mutant (SLT1) for 10 min before being fixed, permeabilized and subjected to PLA using cPLA₂α and AnxA1 antibodies. Representative images taken by a LSM780 confocal microscope are shown. Nucleus is stained by DAPI in blue, scale bar 20 µm. b Quantification of experiment as described in a, presented as % of control (n = 5 independent experiments, mean values +SEM, *p < 0.05). c HeLa cells were treated with 1 µg/ml StxB-S2 or 1.5 µg/ml SLT1 for 10 min and the cell lysates were subjected to immunoprecipitation with a cPLA₂α antibody, followed by immunoblot analysis using AnxA1 and cPLA₂α antibodies. Non-specific rabbit IgG antibody was used as negative control. The levels of AnxA1 and cPLA₂α in the input lysates are shown in the two lower panels. d Quantification of experiments as described in c, with AnxA1 levels normalized to cPLA₂α levels and presented as percentage of control (n = 3 independent experiments +SEM, *p < 0.05)