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. 2015 Aug 23;73(4):869–881. doi: 10.1007/s00018-015-2027-7

Fig. 2.

Fig. 2

Vps35 colocalizes with NS5A. Cured OR6, OR6 cells (a), Huh 7.5.1 cells or JFH1-infected Huh 7.5.1 cells (b) were fixed and immunofluorescently labeled for NS5A and Vps35. DAPI marked nucleus. Colocalization was visualized by confocal microscopy. Areas of overlap are pseudocolored white in the merge and enlarge panels using an ImageJ colocalization highlighter plugin. Scale bars 10 μm. c, d Cured OR6, OR6 cells (c), Huh 7.5.1 cells or JFH1-infected Huh 7.5.1 cells (d) were subjected to Western blotting for analysis