Fig. 6.

Galectin binding to human neuroblastoma cells and functional competition between galectins in cell growth regulation. a Radioiodinated homodimeric galectin-1 (filled circle solid line) and chimera-type galectin-3 (open triangle dashed line) bind with similar affinity to human neuroblastoma cells, whereas proteolytic removal of the N-terminal stalk section from galectin-3 impairs binding (open circle dotted line); inset binding curves. b Reduction of cell surface binding of labeled galectin-1 by increasing concentrations of label-free galectin-3. c Impact of galectin-1 on cell growth (filled circle) relative to the mock-treated control (open square) and the neutralizing effect of galectin-3 applied at a 10-fold excess relative to the concentration of galectin-1 (open triangle) (from Figs. 6–8 in [201], extended by Fig. 2E, F in [192])