Fig. 2.

PCR validation of a gRNA expression carrying 10 expression cassettes. a Schematic illustration of the universal PCR and guide specific PCR for screening of transformed E. coli colonies. F1 (U6-F) and R1 (Scr-R) are used for the universal PCR (Supplementary Table 1). Guide specific PCRs are using the pair sense and antisense gRNA oligonucleotides of the two adjacent gRNAs. b An example of the universal PCR results for a multiplexed gRNA array containing 10 expression cassettes. c An example of the 9 guide RNA specific PCRs for a multiplexed gRNA array containing 10 expression cassettes