Table 1.
Experiment | Probe | Transcript/s | Loc | Sequence |
---|---|---|---|---|
qPCR analysis | TAF1_SYBR_Forward | NM_001286074 | Exon 32 | AGAGTCGGGAGAGCTTTCTG |
NM_004606 | ||||
TAF1_SYBR_Reverse | NM_138923 | Exon 33 | CACAATCTCCTGGGCAGTCT | |
Microarray | A_23_P11237 | NM_001286074 | Exon 38 | GCATGCTTCAGGAGAACACAAGGATGGACATGGAAAATGAAGAAAGCATGATGTCCTATG |
NM_004606 | Exon 37 | |||
NM_138923 | Exon 37 | |||
Microarray | A_32_P192615 | NM_001286074 | Exon 39 (UTR) | GGCTGAGATGAGACTGAAAGATGGGCAGGAAGTATATCATCACAAGCTTTGTGTTTGATG |
NM_138923 | Exon 38 (UTR) | |||
Microarray | A_33_P3353111a | ENST00000373775 (nonsense mediated decay) | Exon 15 (UTR) | TCTTTGTGACTGGTTACTTCATCCAGGGGACCAATTTCTCTCTGGAATATTAGTCCCGCA |
ENST00000462588 (untranslated) | Exon 11 |
NM_001286074, NM_004606, and NM_138923 are the three full-length and most abundant transcripts of TAF1
aTwo examples of alternatively spliced untranslated transcripts interrogated by A_33_P3353111 are given; this probe was eventually filtered out of the analysis due to low expression