Fig. 5.

shRNA-mediated silencing of Katnal2 causes a battery of aberrant phenotypes (I). In all images, α-tub labeling is in red, γ-tub in green and DNA in blue. a1–a4 IF in silenced clone 2.43 shows excessive supernumerary centrioles (yellow arrowheads and square) in an interphase cell. The inset in a2 shows the detail of a cluster of amplified centrioles (contained in the yellow square) at higher magnification. b1–b4 The presence of supernumerary centrioles in silenced cells induces the formation of multipolar mitotic spindles: a cell in prometaphase with eight centrioles and a multi-polar spindle as an example. c1–c4 The depicted silenced cell shows mis-aligned chromosomes on the metaphase plate. Scale bars 10 μm in a1–a4; 3 μm in inset of a2; 5 μm in b and c series