Fig. 1.

Genome editing of TYR gene via the Cas9/gRNA system. a Schematic diagram of the four sgRNA target sites at the tyrosinase (TYR) gene locus. The CDS region is indicated by blue rectangles. SgRNA target sites are indicated by black arrow. SgRNA1and sgRNA2 are located upstream of the TYR locus. SgRNA3 and sgRNA4 are located in the 3′ UTR. 1/2/3 F and 1/2/3 R represent the PCR primer pairs used for mutation detection. The scale bar represents 10 kb. b Constructs and schematic illustration of the Cas9/gRNA system used in this study. c Target sequence of the four sgRNA used in this study