Fig. 4.

PCR and T-cloning sequencing of the target site for each sgRNA in founder rabbit. a Mutation detection of sgRNA1 and sgRNA2 in founder rabbit by PCR. Primers TYR-2F/2R were used for mutation determination. M DNA marker, WT wild-type control. b Mutation detection of sgRNA3 and sgRNA4 in founder rabbit by PCR. Primers TYR-3F/3R were used for mutation determination. M DNA marker, WT wild-type control. c T-cloning sequencing of the target sites of sgRNA1 and sgRNA2 in founder rabbits. PAM sites are underlined and highlighted in red; target sequences are green; deletions (−) and insertions (+) are shown. WT wild-type control. d T-cloning sequencing of the target sites of sgRNA3 and sgRNA4 in founder rabbits. PAM sites are underlined and highlighted in red; target sequences are green; deletions (−) and insertions (+) are shown. WT wild-type control