Fig. 5.

Phenotype identification of TYR mutated rabbits. a Expression of TYR gene was determined by qRT-PCR. WT wild-type control, NW New Zealand white rabbit. The data were analyzed by t tests using Graphpad Prism software. A probability of P < 0.05 was considered statistically significant. *P < 0.05; **P < 0.01; ***P < 0.005. ns not significant, WT wild-type control. b Western blot and gray-scale analysis of the expression of TYR protein. The image was quantified using ImageJ software (NIH) and all the data are expressed as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.005. WT wild-type control. c H&E staining of the irides and skin from the WT and TYR mutated rabbits. The arrows indicate the melanin in the iris and the basal layer of the epidermis. WT wild-type control