Table 1.
Generation of genetically targeted rabbits using CRISPR/Cas9
| Recipients | gRNA/Cas9 mRNA (ng/uL) | Embryos injected | Embryos transferred (% microinjected) | Pregnancy | Pups obtained (% transferred) | Pups with mutations (% pups) | Pups with large fragment deletion (% pups) | Pups with color change |
|---|---|---|---|---|---|---|---|---|
| 1 | 25/100 | 40 | 30 (75 %) | No | ||||
| 2 | 25/100 | 45 | 40 (89 %) | Yes | 8 (20 %) | 6 (75 %) | 1 (16.7 %) | 1 |
| 3 | 25/100 | 40 | 34 (85 %) | No | ||||
| 4 | 25/100 | 37 | 30 (81 %) | Yes | 5 (16.7 %) | 5 (100 %) | 1 (20 %) | 1 |
| 5 | 25/100 | 40 | 35 (88 %) | Yes | 4 (11.4 %) | 4 (100 %) | 1 (25 %) | 1 |