Extended Data Fig. 1. Single-cell and single-nucleus skeletal muscle aging atlas.
a, Dot plot showing marker genes for major cell types in human skeletal muscle aging atlas. The size of the dot represents the proportion of cells expressing a gene. Colour denotes the scaled expression level. b, c, UMAP visualisation of human aging cell atlas coloured according to age (b) and batch: cells or nuclei (c). d, Full version of the plot in Fig. 1d, taking into account 10x chemistry (Methods). e-g, Box plots illustrating proportions of each cell type in every biological replicate (tissue piece) for scRNA-seq (Cells) vs. snRNA-seq (Nuclei) data (averaged across different technical replicates, 15 nuclei vs. 12 cells replicates). Samples containing less than 1000 cells were excluded. The box boundary extends from the 1st quantile (25 percentile) to the 3rd quantile (75 percentile), horizontal line represents median, ‘whiskers’ extend to points that lie within 1.5 IQRs of the lower and upper quartile, observations outside this range are considered ‘outliers’ and marked with a cross. Mann-Whitney-Wilcoxon two-sided test with Benjamini-Hochberg correction was used to quantify the change between cells and nuclei, *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001, ****, p ≤ 1.00e-04, see Source Data for exact p values.