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. 2024 May 21;15:4339. doi: 10.1038/s41467-024-48313-1

Fig. 3. Members of RLCK-VII-6, -7, and -8 differentially contribute to ROS accumulation in N. benthamiana induced by various ExIPs.

Fig. 3

N. benthamiana:Cf-4 rlck-vii-6, rlck-vii-7, and rlck-vii-8 homozygous knock-out lines show a reduced ROS production when compared to N. benthamiana:Cf-4 (the positive control), after treatment with 0.1 μM Avr4 protein (a–c), 0.1 μM flg22 (d–f), and 10 μM chitin (g–i). ROS production is expressed as RLUs, and the data are represented as mean + SEM (n = 12 for Avr4 treatment, n = 8 for flg22 and chitin treatments). All experiments were repeated at least three times with similar results, and representative results are shown. Source data are provided as a Source Data file.