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Evaluating methionine bioavailability through growth curves of methionine-auxotroph ΔmetABJ E. coli in the spent medium of each indicated strain. (Top) Illustration of the experimental scheme employed to assess the bioavailability of methionine in the medium post-growth of each strain. (Bottom) Growth curves of the auxotroph E. coli ΔmetABJ in M9 minimal medium. The medium was supplemented with spent and filtered medium following the growth of the indicated strains.
