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. 2024 Apr 17;300(5):107301. doi: 10.1016/j.jbc.2024.107301

Figure 4.

Figure 4

Effects of ETC perturbations on CoQ redox state.A–C, knockdown of the mitochondrial complex I subunit NDUFS3 using two different shRNAs (#1 and 2), activated glucose consumption relative to scrambled control HT29 cells (A), affected the distribution of the oxidized and reduced CoQ10 pools (B), and the CoQ redox status (C) (n = 3 or 4). D–F, knockdown of the mitochondrial complex II subunit SDHA using two shRNAs (#1 and 2), did not have a sizeable impact on glucose consumption (D), or the distribution of the oxidized and reduced CoQ10 pools (E), and the CoQ redox status (F) (n = 4). G–I, 143B CytB cybrids showed increased glucose consumption (G), decreased total CoQ10 levels (H), and a large reductive shift in the CoQ10 pool (I) relative to wild-type 143B cybrids (n = 4–6). J, HT29 cells expressing LbNOX in the mitochondrion but not in the cytosol exhibited an oxidative shift in the CoQ10 pool.